Enhanced TDS
Knowde-enriched technical product data sheet
Identification & Functionality
- Pharma & Nutraceuticals Functions
- Technologies
- Product Families
Features & Benefits
- Product Features
Gene: Protocatechuate 3,4-dioxygenase gene cloned from Acinetobacter baumannii
Production: Purified from the extract of transformed E.coli by conventional chromatographies
Purity: Single major band on SDS-PAGE (low contaminants from host E.coli)
Application: Useful for removal of protocatechuate derived from choline esterase determination
Stability: Wide range of pH stability from 5 to 10
Applications & Uses
- Markets
- Applications
- Preparation
Product Code：rPCO-04 Solution
IU per 1 ml solution is ＞80 units
Properties
- Physical Form
- Typical Properties
Value Units Test Method / Conditions Specific Activity 3.0 IU/mg protein - - Contaminants
Value Units Test Method / Conditions NADH oxidase max. 0.01 % - Alkaline phosphatase max. 0.002 % - pH stability pH 5.0 - 10.5（11, 1 week） - - Thermal stability （pH 8.0, 15 min） max. 60 ℃ - Optimum pH 9.0 - - Optimum temperature 65.0 ℃ - Km value 28（Protocatechuate） μmol/L - Molecular Weight (SDS-PAGE, α subuit） 28.0 kD - Molecular Weight (SDS-PAGE, β subunit） 24.0 kD -
Technical Details & Test Data
- Reference Data
- Reaction Equation
Protocatechuate + O2 ＝ 3-Carboxy-cis,cis-muconate
- Assay Procedure
Spectrophotometric Method
- Wavelength；290 nm
- Light path length；1 cm
- Temperature；37℃
- Pipette the following reagents into a cuvette
- 3.00 mL Tris-acetate buffer（50 mmol/L, pH 7.2, 37℃） containing Protocatechuate（0.4 mmol/L）
- 0.02 mL rPCO（1.2～1.6 IU/mL）
Packaging & Availability
- Packaging Type
- Packaging
OYC No. Package 46852004 300 units 46852904 Bulk
Storage & Handling
- Storage Condition
1 - 10°C (do not freeze)