M.C.Biotec Quaternium-73

Compliant with Japanese Quasi Drugs to be used in anti-acne products; China Compliant

1.   ANTIBACTERIAL ACTIVITY

Quaternium-73 shows extremely strong antibacterial activity at very low concentration. It damage the  microorganisms by causing collapse of cell membrane and cell wall and intracellular degradation.

Quaternium-73 is similar to cyanine dyes. Even a single Quaternium-73 molecule also has anti-bacterial activity, which is the reason why Quaternium-73 is still efficient even with an extremely low dose.
1.1 Fast and deep effect on anti-acne
Quaternium-73 has an excellent effect on Propionibacterium acnes. It is commonly used in Japanese over-thecounter (OTC) as an anti-acne ingredient. Clinical research shows that it has a MIC = 0.00002% for acne bacilli, and the rash can be reduced by 50% after two weeks of use.


Clinical Test:
Within two weeks, it reduced 50% spots Testing program: Carry out test in 38 testees with acne problems Method: Wash face 3 times every day with emulsion containing 0.001% of Quaternium-73 and smears twice a week with cream containing 0.005% of Quaternium-73.


1.2 Bactericidal Activity
The Quaternium-73’s bactericidal ability allows it to be used as a preservative. In vitro test data shows that in term of anti-bacteria and anti-fungi of various kinds, Quaternium-73 is more effective than paraben methyl ester. Therefore, just adding a small amount of paraben in the formulation containing Quaternium-73 is enough to achieve an antibacterial effect.

● Quaternium-73 can be used alone (see the picture below, the number of E.coli contain ed in 0.002% quaternium-73)

● Quaternium-73 can compensate for the methylparaben (Adding 0.002% quaternium-73 can lead to an increase of anti-E.Coli activity of 0.005% methylparaben)

● When mixed with polyols, Quaternium-73 can completely replaced parabens as a preservative

1.3 Inhibits bacteria and fungi with an extremely low dose of use ( In vitro test )
In vitro test (see table below, agar plate dilution) proves that the extremely low dose of use of Quaternium-73 can also inhibit a wide range of bacteria and fungi. The lowest concentration of Quaternium-73 used to inhibit bacterial growth is called the Minimum Inhibitory Concentration (MIC).

2. Strong inhibition of melanin
In vitro test indicates that: 0.000003% Quaternium-73 can suppress 50% melanin formation 0.00001% Quaternium-73 can suppress 83% melanin formation. In contrast, 0.07% kojic acid can inhibit 100% melanin formation.


 

• Human body patch test ( human body, 0.2%, 48 hours ): negative
• Initial skin irritation ( rabbit, 0.2%, 24 hours ): negative
• Sustained skin irritation ( rabbit, 0.01%, 90 day ):negative
• Eye irritation ( rabbit, 0.0007%, 24 hour ): negative
• Skin sensitization ( Guinea pig, 0.2%, maximum test ): negative
• Phototoxicity ( rabbit, 0.2%): negative
• Photosensitization ( Guinea Pig, 0.2%, Adjuvant-Strip Method ): negative
• Induced gene mutation (10μg, Ames test, ±S9 mix): negative
• Acute Toxicity LD 50 ( mouse )：325 mg/kg; LD50 oral rat: 460mg/kg

Stored in a cool, dry, dark place. Special care is taken to prevent the powder from diffusing into the air. Wear a mask to avoid inhaling powder. After use please wash your body, hands, mouth and face thoroughly.