ProBiotein® Multi-Prebiotic and Postbiotic

Introduction

As the poultry industry continues to remove anitbiotic growth promoters from ever being introduced during rearing, alternatives continue to be explored that can improve performance and promote the health of the bird, including through gastrointestinal modulation. Previously, yeast-derived fermentation products have previously been shown to potentially modulate the gastrointestinal microbiota composition, which has implications on bird health and development.

Objective

The objective of the current research was to determine the effects on the ileal microbiota development in broilers fed increasing levels of a yeast-derived fermentation product, ProBiotein®.

Hypothesis

We hypothesized that supplementating ProBiotein® would beneficially alter the ileal microbial ecology.

Methods

• 400 male Ross 308 chicks were placed into batteries, where 10 birds each were placed into a total of 40 pen between two batteries. On d 14, birds were moved to 8 floor pens, where all chicks receiving the same treatment from each battery (4 pens) were combined into a single floor pen such that the same blocking design was followed. Birds were fed an industrystandard least cost diet supplemented with the following treatments: 0.00% PB + 0.75% sand; 0.20% PB + 0.55% sand; 0.50% PB + 0.25% sand; 0.75% PB.
• On d 14 and 42, 10 birds per treatment were euthanized and their ileal contents were expressed. The genomic DNA were extracted and samples were sequenced on an Ilumina MiSeq platform. Data were analyzed in QIIME2 for the main effects of treatment and day, and the interaction between. Alpha diversity was analyzed through ANOVA with pairwise differences separated through Kruskal-Wallis. Beta diversity was analyzed through ADONIS, with pariwise differences separated through ANOSIM.

Results

Faith Group Significance

Shannon Group Significance

Jaccard

Weighted UniFrac

Eighteen taxa were significantly different in relative abundances, including Lactobacillus, Butyricicoccus, Peptostreptococcus, and Weisella.

Lactobacillus

Butyricicoccus

Peptostreptococcus

Weissella

Conclusion

Day was a driving factor in the development of the ileal microbiota composition, but these differences were altered and magnified due to treatment supplementation level. Taxonomical compositions varied due to treatment, with differing relative abundances of previously reported beneficial fermentative taxa, such as Lactobacillus, Peptostreptococcus, Butyriciccocus, and Weisella. Though the overall relationships between ileal microbiota and the host have not been fully elucidated, but have been suggested to promote the immune system's development and alter growth performance, The current results suggest ProBiotein® modulates the microbiota composition and development and may be a beneficial feed amendment for inclusion in poultry diets.

Introduction

• 9 million Americans suffer from foodborne illness per year¹
  • 40% attributed to land animals
• Salmonella enterica are among the most isolated foodborne pathogens from livestock^1,2,3
• Modulation of the broiler gastrointestinal (GIT) microbiota through the use of in-feed supplements has been an effective measure to reduce foodborne pathogens⁴

Note:

¹Painter et al., 2013
²CDC, 2016
³FSIS, 2014, 2016
⁴Feye et al., 2021

Objective

Evaluate the anti-Salmonella effect of varying levels of an in-feed supplement, a yeast fementate (YF), ProBiotein®, in an in vitro GIT model.

Material & Methods

Sampling

Statistical Analyses

• Data were analyzed in JMP® Pro 15
• pH data
  • Mixed effect model (random effect=trial)
• Microbiological data
  • Mixed effect model (random effect=trial)
• Pairwise differences were determined using Tukey’s HSD with a significance of P ≤ 0.05

Results

Figure 1. Effect of a yeast fermentate on the in vitro intestinal pH (P < 0.05)

• Time impacted pH more than treatment
• Numerically, 1.25% had lowest pH at 24 h (5.35 pH)
• Treatment 1.25% (-3.604 Log CFU/mL) had greater change in Salmonella than 0.25% (-1.748)

Figure 2. Effect of a yeast fermentate on Salmonella change (P < 0.05)

Conclusion

These results demonstrate the potential of ProBiotein® as an in-feed anti-Salmonella broiler supplement.

Introduction

• 9 million Americans suffer from foodborne illness per year¹
  • 40% attributed to land animals
• Salmonella enterica are among the most isolated foodborne pathogens from livestock^1,2,3
• Modulation of the broiler gastrointestinal (GIT) microbiota through the use of in-feed supplements has been an effective measure to reduce foodborne pathogens⁴

Note:

¹Painter et al., 2013
²CDC, 2016
³FSIS, 2014, 2016
⁴Feye et al., 2021

Objective

Evaluate the anti-Salmonella effect of varying levels of an in-feed supplement, a yeast fementate (YF), ProBiotein®, in an in vitro GIT model.

Material & Methods

Sampling

Statistical Analyzes

• Data were analyzed in JMP® Pro 15
• pH data
  • Mixed effect model (random effect=trial)
• Microbiological data
  • Mixed effect model (random effect=trial)
• Pairwise differences were determined using Tukey’s HSD with a significance of P ≤ 0.05

Results

Figure 1. Effect of a yeast fermentate on the in vitro intestinal pH (P < 0.05)

• Time impacted pH more than treatment
• Numerically, 1.25% had lowest pH at 24 h (5.35 pH)
• Treatment 1.25% (-3.604 Log CFU/mL) had greater change in Salmonella than 0.25% (-1.748)

Figure 2. Effect of a yeast fermentate on Salmonella change (P < 0.05)

Conclusion

These results demonstrate the potential of ProBiotein® as an in-feed anti-Salmonella broiler supplement.