Arcolys®

Depigmentation of hair is due to a lack of melanin and, in particular, a decrease of melanogenesis activity (reduction of tyrosinase activity, reduction of melanosome number and defect in melanosome transfer) and a reduced number of differentiated and functional melanocytes. Oxidative stress also contributes to numerous process, reducing hair growth and hair graying.

• Young Hair has active melanocyte stem at the bulbe area. It is under a low oxidative stress.
• Aged Hair follicle is under a high oxidative stress with high concentration of H2O2. DNA repair are also low.

• ARCOLYS® 1% (N=21) versus placebo (N=23)
• Twice daily applications during 154 days (5 months)
• Women (29) and Men (15) with 20% to 50% of white hair
• Mean age: 49 years old

Evalution Parameters

• Visual scoring of white hair proportion: at M0, M2.5 & M5
• Density of white hair (number.cm-2): at M0, M2.5 & M5
• Self-assessment: at M5
• Psychological evaluation: at M0 and M5

1. Self-esteem (Rosenberg, 1965)
2. Emotional valence (Brief Mood Introspection Scale: Mayer and Gaschle, 1988)

Arcolys® Reduces Ros Production In Dermal Papilla Cells

Dermal papilla cells present in the hair follicle bulb play an essential role in the regulation of active melanocyte renewal. Accumulating intracellular ROS affect their activity

     RELATIVE QUANTIFICATION OF ROS IN HDPC (% OF CONTROL WITH H2O2)

Incubation of Human dermal papilla cells (HFDPC) with ARCOLYS® during 24 hours. Oxidative stress was applied: 250μM H2O2 during 30 min. Intracellular ROS were measured using a fluorogenic probe (2,7-DCDHF-DA) (n=3).
*: p< 0.05

Arcolys® Limits Oxidative Stress Environment, Improving Hair Pigmentation.

Arcolys® Enhances The Expression Of Antioxidant In Melanocytes

NRF2 is key transcription factor of the antioxidant responses via regulating antioxidant gene expression, including HMOX1 and SLC7A11, the latest promoting glutathione biosynthesis, leading to protection from oxidative stress.

Incubation of melanocyte cells with ARCOLYS® during 48 hours. Oxidative stress after 24 hours of treatment : 250μM H2O2 during 2 hours. mRNA expression of NFR2, HMOX1, SLC7A11 gene using RT-qPCR on pooled culture triplicates.
NFR2 : nuclear factor erythroid 2–related factor, HMOX1: Heme oxygenase 1, SLC7A11: Solute Carrier Family 7 Member 11

Arcolys® Improves Antioxidant Properties, Limiting Hair Whitening.

Arcolys® Stimulates Tyrosinase Activity

Tyrosinase is a key enzyme for the biosynthesis pathway of melanin pigment, which is the most important determinant of the color of skin and hair.

Incubation of melanocyte cells with ARCOLYS® during 48 hours. Oxidative stress after 24 hours of treatment : 250μM H2O2 during 2 hours. mRNA expression of TYR gene using RT-qPCR on pooled culture triplicates. Arcolys® Increases Tyrosinase Gene Expression, Promoting Melanogenesis.

Arcolys® Stimulates Tyrosinase Activity

Aging is associated with reduced number of differentiated and functioning hair bulb melanocytes, and decreased tyrosinase activity.

Incubation of melanocyte cells with ARCOLYS® during 72 hours (n=3). Oxidative stress after 72 hours of treatment : 5mM H2O2 during 30 minutes. Enzymatic activity evaluated by optical density (OD) at 540nm after incubation with L-DOPA substrate.
**p< 0.01

Arcolys Increases Tyrosinase Activity And Thus Pigmentation Process.

Arcolys® Stimulates Melanin Synthesis

Melanin is responsible for the color of eyes, hair. It is formed through a series of reactions involving the amino acid tyrosine in the presence of the enzyme tyrosinase.

MELANIN SYNTHESIS

Incubation of melanocyte and keratinocyte co-culture with ARCOLYS® or L-Tyrosine during 10 days. Oxidative stress at day 0, 3 and 7: 250 μM H2O2 during 2 hours. Intracellular melanin content evaluated by optical density (OD) at 405nm (n=3).

**p< 0.01, ***p< 0.001

Arcolys® Enhances Melanin Synthesis And Thus May Reverse Hair Pigmentation Loss.

**p<0.01, ***p<0.001, **** p<0.0001 vs M0, ns: not significant

• Arcolys® 1% Significantly Decreases White Hair Density After 2.5 Months And Even More After 5 Months Of Application
• Arcolys®: More Than A Repigmentation Active, A Systemic Approach Of Hair

Arcolys® Boosts Self-Esteem 

Graying hair can result in a loss of self-esteem and anxiety, and other emotional issues.

                                                 ARCOLYS® IMPROVES SELF-ESTEEM.

Arcolys® Decreases Unpleasant Emotions

Emotional valence is a dimension continuum of pleasant and unpleasant emotions, which can be measured using the Brief Mood Introspection Scale.

ARCOLYS® SIGNIFICANTLY DECREASES UNPLEASANT EMOTIONS, INDUCING A HIGHER SELF-ESTEEM

Cutaneous irritation: Very good skin compatibility
Sensitization: Very good skin compatibility
Eye irritation: Slightly irritant
Phototoxicity test: Not phototoxic
Mutagenicity test: Not mutagenic

Keep in a dark place, in the original packaging, at an ambient temperature between 15°C and 25 °C.