MIRASIL™ ADMH B 100

Free Radical Scavenger

The aim of this study is to evaluate the antioxidant properties of a cosmetic raw material by means of its anti-radicals features. Antiradical activity assay is based on the reduction of 1,1-diphenyl-2-picrylhydrazyl (DPPH). Due to the presence of an odd electron, it gives a strong absorption maximum at 515 nm.

Protocol

Experimental protocol required the determination and the quantification of antioxidant activity of tested sample by means of evaluation of its scavenging properties against synthetic radical DPPH.

Tested samples were added to cups containing a DPPH solution and the solution absorbance variation at 515 nm was measured during 5 minutes.

Results

For each tested sample concentration, reduced DPPH concentration after monitored period was calculated. These quantities were plotted with sample concentrations and EC50 parameter was calculated (EC50 is the sample concentration which reduces by 50% the initial DPPH concentration).

Anti-Radical Power ARP (ARP) was calculated too as the inverse of EC50, expressed in %.

The Ferrin Reducing Antioxidant (FRAP) parameter is a direct measure of the total reductive power of a matrix and an indirect index of the capability of the product to protect against the oxidative damage.

Conclusion

Experimental data show that tested product has a scavenging activity against synthetic radical DPPH at all of tested concentrations and with a dose-dependence feature. The product added to a base cream maintains its activity that is proportional to its final concentration.

Experimental data show that tested product has a reducing activity against the used oxidant agent at all of tested concentrations and with a dose dependence feature. The product added to a base cream maintains its activity that is proportional to its final concentration.