Cola® Lipid C

Cola® Lipid C is a safe ingredient for formulations, providing high performance with minimal or no irritation results.

Eye Irritation

MatTek: EpiOcular™ Tissue Model In Vitro Toxicity Testing System: Results indicate ‘non-irritating’ classifi cation.

Skin Irritation

MatTek: In Vitro EpiDerm™ Skin Irritation Test (EPI-200-SIT) (OECD 439): Results indicate ‘non-irritating’ classifi cation.

Acute Skin Irritation

48 Hour Occlusive skin patch test: On human volunteers - 53 Test Subjects: no visible skin reaction, no potential for dermal irritation.

Skin Sensitization

Repeat Insult Patch testing (HRIPT): no potential for dermal irritation or allergic contact sensitization.
Method OECD Test Guideline 442c - Direct Peptide Reactivity Assay (DPRA) and Method 442D - the KeratinoSens test method: Cola®Lipid C is classifi ed as a non-sensitizer with no or minimal reactivity (40% active).

Biodegradability

OECD 301 (301D) Ready biodegradability test in an aerobic aqueous medium: Sample exceeds 60% biodegradability requirement in seven days. Cola®Lipid C is readily biodegradable.

80% Biobased

Certified 80% natural carbon via independent testing through the USDA Biobased program, allowing for a wide variety of NGO certifications.

Cola® Lipid C can be used to reduce or eliminate the use of classical preservatives to achieve self-preservation strategies with improved skin health benefits. Its performance is not adversely affected by solution pH, amphoteric or nonionic surfactants, or typical preservative deactivators.

Preservative Challenge Testing

Test material was diluted to a fi nal test concentration of 1.0% in phosphate buff ered saline. Representative aliquots of test preparation were inoculated with separate mixed cultures of bacteria and fungi. Plate counts to determine survivors were performed at 0 time and after 1, 3, 7 and 14 after inoculation. Sample was inoculated at both 0 time and 7 days. Results are presented as the number of surviving organisms present at each time interval per gram of material tested. Inoculum levels were ~106 per gm for the mixed bacteria and ~105 per gm for the mixed fungi.

SAMPLE: Cola® Lipid C Lot # 40344H13 - 1.0 % in PO4 Buffer

Inoculum

Mixed Bacteria: Pseud. aeruginosa (ATCC 15442); B. cepacia (ATCC 25416); E.coli (ATCC 8739 or 11229); S. aureus (ATCC 6538).

Mixed fungi: A. brasilienis (niger) (ATCC 16404); C. albicans (ATCC 10231); Penicillium luteum (ATCC 9644) or Penicillium levitum (ATCC 10464).

Results

• Cola® Lipid C test sample passed the modified Accelerated Double Challenge Preservative Testing protocol.
• At a 1.0% concentration, these material demonstrate an excellent rate of kill and preservative capacity with both bacteria and fungi being eliminated within 24 hours of each inoculation.
• Cola® Lipid C is not EPA registered as an antimicrobial agent.